Establishment of reference intervals for pediatric complete capillary blood counts: A multicenter study in Beijing
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August 16, 2020

Establishment of reference intervals for pediatric complete capillary blood counts: A multicenter study in Beijing

By Alexis

Introduction: clinical reference intervals represent the normal range of clinical parameters to distinguish between healthy and sick people, and they showed some variation among different populations. Many still lack a reference interval for the population of children in China. Thus, the purpose of this study was to develop and validate a pediatric reference intervals for capillary blood cell count.


Methods: A total of 9942 children were enrolled from 10 medical institutions in Beijing, China, for (CCBC) complete blood capillaries value in accordance with the guidelines issued by the EP28-A3C Clinical and Laboratory Standards Institute.


Results: Pediatric reference intervals for 17 CCBC parameters established for children aged 6 months to 7 years. The number of red blood cells and red blood cell distribution width is generally higher in males than females, and the average living cell hemoglobin and mean cell volume is higher in women than in men. Red blood cells, hemoglobin, hematocrit, and the percentage of neutrophils increased while the percentage of lymphocytes decreased with age. The overall trend for each interval relative references together in groups of different ethnic groups and regions in the world, but with variations in the upper and lower limits, which confirms the existence of racial and geographic differences. Further validation with 508 healthy subjects demonstrated that the proportion is verified to be within 90.9% -100% of the reference interval.


Conclusion: This study offers a local reference interval for values ​​CCBC for the pediatric population in Beijing, China, and thus provide basic criteria for the diagnosis, treatment, and health assessment of childhood diseases in China.


Small cell neuroendocrine carcinoma (SCNC) larynx are very rare. Primary small cell neuroendocrine carcinoma is less than 0.5% of all cancers of the larynx. Tumors can occur in any laryngeal subsite, while supraglottis is the most common. Patients usually complaint with hoarseness and neck masses.

This kind carcinoma diagnosis relies strictly on histopathology and immunohistochemistry testing. SCNC larynx prognosis is generally poor. Here, we report a case of a patient 55-year-old male who presented with poor SCNC distinguished from the supraglottic larynx and neck masses.

Establishment of reference intervals for pediatric complete capillary blood counts: A multicenter study in Beijing
Establishment of reference intervals for pediatric complete capillary blood counts: A multicenter study in Beijing

miR-429 inhibits the development of osteosarcoma by targeting HOXA9 through pressing the Wnt / β-catenin signaling pathway

steosarcoma (OS) is the most common malignant cancer diagnosed of bone that occurs in adolescents and children. Installation of a number of studies have shown that miRNAs is increasingly playing a fundamental role in the development of the OS.

Thus, the biological function of miR-429 in the development of OS explored. The results of RT-qPCR revealed that miR-429 were downregulated in the network OS and OS cell line (MG-63, U2OS, sauce-2) while homeobox A9 (HOXA9) markedly increased. Additionally, HOXA9 confirmed as a direct target of miR-429 by using a luciferase reporter assay.

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BrightGreen 2X qPCR MasterMix-ROX
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BT11001 250rxn
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BT11101 250rxn
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9-31045
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9-31046
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BT11002 250rxn
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BT11102 250rxn
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3326 1/EA
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31044-1 1kit
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2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
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2xEs Taq Master Mix (with Dyes)
W0690-1 NULL
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W0690-5 NULL
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HY-K1004 100 μL
EUR 113.00

qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials
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EUR 138.00

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EUR 523.00

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EUR 138.00

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S175L 500 rcs (12,5 ml)
EUR 523.00

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S215 100 rcs (2,5 ml)
EUR 159.00

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S215L 500 rcs (12,5 ml)
EUR 523.00

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S220 100 rcs (2,5 ml)
EUR 159.00

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S220L 500 rcs (12,5 ml)
EUR 612.00

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S225L 500 rcs (12,5 ml)
EUR 612.00

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QPCR002-L 4X1.25ml
EUR 252.71
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AZ-2301 200 Rxn
EUR 179.00

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AZ-2305 500 Rxn
EUR 320.00

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AZ-2320 2000 Rxn
EUR 996.00

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AZ-2325 5000 Rxn
EUR 2348.00

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AZ-2401 200 Rxn
EUR 179.00

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn
AZ-2405 500 Rxn
EUR 320.00

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn
AZ-2420 2000 Rxn
EUR 996.00

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn
AZ-2421 5000 Rxn
EUR 2348.00

Bio-Star Mastermix with SYBR (2X) no dye
Y210 100 rcs (2,5 ml)
EUR 89.00

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)
31041-1 5x1mL
EUR 272.00
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)
31041-20mL 2x10mL
EUR 494.00
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)
31041-T 1mL
EUR 114.00
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Deoxy+ SYBR Green OneStep qRT-PCRKit wi ROX, 100 preps
FYT504-100P 1 vial Ask for price

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Green qPCR SuperMix
20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
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qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S180 100 rcs (2,5 ml)
EUR 159.00

qPCR Master Mix E4 (SafeLine: EvaGreen, dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials
S180L 500 rcs (12,5 ml)
EUR 612.00

qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials
S185 100 rcs (2,5 ml)
EUR 159.00

qPCR Master Mix E6 (EvaGreen, dUTP, UNG, 100 nM LOW ROX), packed in 1,25 ml vials
S185L 500 rcs (12,5 ml)
EUR 612.00

2×Taq PCR Master Mix(with dye)
K1034-1 1 ml
EUR 73.00

2×Taq PCR Master Mix(with dye)
K1034-100 100×1 ml
EUR 792.00

2×Taq PCR Master Mix(with dye)
K1034-20 20×1 ml
EUR 282.00

2×Taq PCR Master Mix(with dye)
K1034-5 5×1 ml
EUR 119.00

2×Taq PCR Master Mix(with dye)
K1034-50 50×1 ml
EUR 514.00

Jade? Mix-ROX
M1108-500
EUR 441.00

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)
105-520 2 x 1,25 ml
EUR 458.00

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)
105-522 10 x 1,25 ml
EUR 1719.00

One.Step RT-qPCR with EvaGreen / HighROX (2X concentrated)
105-530 2 x 1,25 ml
EUR 427.00

One.Step RT-qPCR with EvaGreen / HighROX (2X concentrated)
105-532 10 x 1,25 ml
EUR 1652.00

ACCUZYME Mix, 2x
BIO-25028 500 Reactions Ask for price

MyTaq Mix, 2x
BIO-25041 200 Reactions Ask for price

MyTaq Mix, 2x
BIO-25041/S Sample Ask for price

MyTaq Mix, 2x
BIO-25042 1000 Reactions Ask for price

MyFi Mix, 2x
BIO-25049 100 Reactions Ask for price

MyFi Mix, 2x
BIO-25049/S Sample Ask for price

MyFi Mix, 2x
BIO-25050 500 Reactions Ask for price

It is identified that miR-429 exhibited a suppressive effect on the development of the OS while HOXA9 show oncogenic function in the development of the OS by using MTT and Transwell test. More importantly, realized that HOXA9 rescue tests can partially cancel the suppressive effect of miR-429 on the OS. Overexpression of miR-429 inhibits the activation of the Wnt / β-catenin signaling pathway. In conclusion, miR-429 suppressed the development of the OS by targeting HOXA9 through the Wnt / β-catenin pathway.

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